RUB » Collaborative Research Centres (SFBs) » SRC642: GTP- and ATP dependent membrane processes

Publications 2017

  • When, how and why? – Regulated proteolysis by the essential FtsH protease in Escherichia coli. Bittner LM, Arends J, Narberhaus F.
    Biol Chem. 2017 May 1;398(5-6):625-635. doi: 10.1515/hsz-2016-0302 (2017)
  • The copper efflux regulator CueR is subject to ATP-dependent proteolysis in Escherichia coli. Bittner LM, Kraus A, Schäkermann S, Narberhaus F.
    Front Mol Biosci. 2017 Feb 28;4:9. doi: 10.3389/fmolb.2017.00009 (2017)
  • Molecular Activity Painting”: Switch-like, light-controlled perturbations inside living cells. Chen X, Venkatachalapathy M, Kamps D, Weigel S, Kumar R, Orlich M, Garrecht R, Hirtz M, Niemeyer CM, Wu YW*, Dehmelt L*.
    Angew Chem Int Ed Engl. 2017 Mar 29. doi: 10.1002/anie.201611432 (2017)
  • Influence of Isoform-specific Ras Lipidation Motifs on Protein Partitioning and Dynamics in Model Membrane Systems of Various Complexity. Erwin, N., S. Patra, M. Dwivedi, K. Weise, and R. Winter.
    Biol Chem. 2017 May 1;398(5-6):547-563. doi: 10.1515/hsz-2016-0289 (2017)
  • Expression and biological functions of the extracellular matrix glycoprotein tenascin-C in the neural stem and glial progenitor cell compartments. Faissner, A., Roll, L. and U. Theocharidis.
    Molecular and Cellular Neuroscience, in press (2017)
  • Common mechanisms of catalysis in small and heterotrimeric GTPases and their respective GAPs. Gerwert, K., Mann, D., and Kötting, C.
    Biol. Chem. 398, 523–533 (2017)
  • Mechanisms of action of Rab proteins, key regulators of intracellular vesicular transport. Goody, R.S., Müller, M.P., and Wu, Y.-W.
    Biol. Chem. 398, 565–575 (2017)
  • Helional-induced activation of human olfactory receptor 2J3 promotes apoptosis and inhibits proliferation in a non-small-cell lung cancer cell line. Kalbe, B., Schulz, V., Schlimm, M., Philippou, S., Jansen, F., Scholz, P., Lübbert, H., Jarocki, M., Faissner, A., Hecker, E., Veitinger, S., Tsai, T., Osterloh, S., and H. Hatt.
    European Journal of Cell Biol, in press (2017)
  • Constantinescu-Aruxandei. Kinetic characterization of apoptotic Ras signalling through Nore1-MST1 complex formation. Koturenkiene A., Makbul C., C. Herrmann, D.
    Biol. Chem. Jan 28. doi: 10.1515/hsz-2016-0291 (2017)
  • Discovery of Novel Cinchona-Alkaloid-Inspired Oxazatwistane Autophagy Inhibitors. Laraia L, Ohsawa K, Konstantinidis G, Robkea L, Wu YW, Kumar K, Waldmann H.
    Angew Chem Int Ed., 56(8):2145-2150. (2017)
  • Next-generation trapping of protease substrates by label-free proteomics. Lindemann C, Thomanek N, Kuhlmann K, Meyer HE, Marcus K, Narberhaus F.
    Methods Mol Biol. in press. (2017)
  • 3D-visualization and quantification of microvessels in the whole ischemic mouse brain using solvent-based clearing and light sheet microscopy. Lugo-Hernandez, E., A. Squire, N. Hagemann, A. Brenzel, M. Sardari, J. Schlechter, E.H. Sanchez-Mendoza, M. Gunzer, A. Faissner and D.M. Hermann.
    J Cereb Blood Flow Metab. 2017 Jan 1:271678X17698970 (2017)
  • Elucidation of Single Hydrogen Bonds in GTPases via Experimental and Theoretical Infrared Spectroscopy. Mann, D., Höweler, U., Kötting, C., Gerwert, K.
    Biophysical J., 1 (112), 66-77 (2017)
  • A PDE6δ-KRas Inhibitor Chemotype with up to Seven H-Bonds and Picomolar Affinity that Prevents Efficient Inhibitor Release by Arl2. Martín-Gago P., Fansa E.K., C. H. Klein, S. Murarka, P. Janning, M. Schürmann, M. Metz, P.I.H. Bastiaens, A. Wittinghofer, H. Waldmann.
    Angew. Chem. Int. Ed., 56, 2423-2428 (2017)
  • Local Mode Analysis: Decoding IR Spectra by Visualizing Molecular Details. Massarczyk,M.,Rudack,T.,Schlitter, J.,Kuhne,J., Kötting,C., Gerwert,K.
    J. Phys. Chem. B, DOI: 10.1021/acs.jpcb.6b09343 (2017)
  • Small Molecule Inhibition of the UNC119-Cargo Interaction. Mejuch T., Garivet G., Hofer W., N. Kaiser, E. K. Fansa, C. Ehrt, O. Koch, M. Baumann, S. Ziegler, A. Wittinghofer and H. Waldmann*.
    Angew Chem Int Ed Engl. 2017 May 4. doi: 10.1002/anie.201701905 (2017)
  • Rheb in neuronal degeneration, regeneration, and connectivity. Potheraveedu VN, Schöpel M, Stoll R, Heumann R.
    Biol Chem. 2017 May 1;398(5-6):589-606. doi: 10.1515/hsz-2016-0312 (2017)
  • The class III phosphatidylinositol 3-kinase Vps34 in Saccharomyces cerevisiae. Reidick, C., Boutouja, F. & Platta, H.W.
    Biol Chem. 2017 May 1;398(5-6):677-685. doi: 10.1515/hsz-2016-0288 (2017)
  • The small GTPases Ras and Rheb studied by multidimensional NMR spectroscopy: structure and function. Schöpel Miriam, Potheraveedu Veena Nambiar, Thuraya Al-Harthy, Raid Abdel-Jalil, Rolf Heumann and Raphael Stoll*.
    Biol Chem. 2017 May 1;398(5-6):577-588. doi: 10.1515/hsz-2016-0276 (2017)
  • ATPdriven processes of peroxisomal matrix protein import. Schwerter, D.P., Grimm, I., Platta, H.W., and Erdmann, R.
    Biol. Chem. 398, 607–624 (2017)
  • Involvement of the guanine nucleotide exchange factor Vav3 in central nervous system development and plasticity. Ulc, A., Gottschling, C., Schäfer, I., Wegrzyn, D., van Leeuwen, S., Luft, V., Reinhard, J. and A. Faissner.
    Biol Chem. 2017 May 1;398(5-6):663-675. doi: 10.1515/hsz-2016-0275 (2017)
  • Interface analysis of small GTP binding protein complexes suggests preferred membrane orientations. Vetter, I.R.
    Biol. Chem. 398, 637–651 (2017)

Publications 2016

  • In vivo trapping of FtsH substrates by label-free quantitative proteomics. Arends J, Thomanek N, Kuhlmann K, Marcus K, Narberhaus F.
    Proteomics 16:3161-3172 (2016)
  • dentification of protein phosphatase 2A as an interacting protein of leucine-rich repeat kinase 2. Athanasopoulos P.S., Jacob W., Neumann S., M. Kutsch, D. Wolters, E.K. Tan, Z. Bichler, C. Herrmann, R. Heumann.
    Biol Chem. 397, 541-554 (2016)
  • ATP-dependent proteases in bacteria. LM, Arends J, Narberhaus F.
    Biopolymers 105:505-517 (2016)
  • Galectin-1 dimers can scaffold Raf-effectors to increase H-ras nanoclustering. Blazevits O., Mideksa Y., Solman M., A. Ligabue, N. Ariotti, H. Nakhaeizadeh, E. Fansa, A.C. Papageorgiou, A. Wittinghofer, M. Ahmadian, and D. Abankwa.
    Scientific Reports 6:24165, DOI: 10.103/srep24165 (2016)
  • Selective chemical labeling of proteins. Chen X, Wu YW.
    Org. Biomol. Chem. 14(24):5417-39. Invited Review in New Talent Theme Issue (2016)
  • Protease-resistant and cell-permeable double-stapled peptides targeting the Rab8a GTPase. Cromm P. M., Spiegel J., P. Küchler, L. Dietrich, J. Kriegesmann, M. Wendt, R. Goody, H. Waldmann, T.N. Grossmann.
    ACS Chemical Biology, 11, 2375-2382 (2016)
  • Neuron-glia interactions in neural plasticity: contributions of neural extracellular matrix and perineuronal nets. Dzyubenko, E., Gottschling, C. and A. Faissner.
    Neural Plasticity, Article ID 5214961, pp. 1-14 (2016)
  • Co-Localisation of synapse marker proteins evaluated by STED-microcopy reveals patterns of synapse distribution in vitro. Dzyubenko, E., Rozenberg, A., Hermann, D.and A. Faissner.
    Journal of Neuroscience Methods 273, 149-159 (2016)
  • Assembly, maintenance and dynamics of peroxisomes. Erdmann R.
    Biochim Biophys Acta 1863(5), 787-9 (2016)
  • Probing Conformational and Functional Substates of Calmodulin by High Pressure FTIR Spectroscopy: Influence of Ca2+ Binding and the Hypervariable Region of K-Ras4B. Erwin, N., S. Patra, and R. Winter.
    Phys. Chem. Chem. Phys. 18; 30020-30028; DOI: 10.1039/C6CP06553H (2016)
  • Lipoprotein Insertion into Membranes of Various Complexity: Lipid Sorting, Interfacial Adsorption and Protein Clustering. Erwin N., Sperlich B., G. Garivet, H. Waldmann, K. Weise, R. Winter.
    Phys. Chem. Chem. Phys. 18, 8954-8962 (2016)
  • PDE&δ-mediated sorting of INPP5E into the cilium is determined by cargo-carrier affinity. Fansa E.K., Kösling S.K., Zent E., A. Wittinghofer and S. Ismail.
    Nature Communications 7:11366, DOI: 10.1038/ncomms11366 (2016)
  • Sorting of lipidated cargo by the Arl2/3 system. Fansa E.K. and A.Wittinghofer.
    Small GTPases 2016, 7,222-230 (2016)
  • Unraveling the phosphocholination mechanism of the Legionella pneumophila enzyme AnkX. Gavriljuk, K., Schartner, J., Seidel, H., Dickhut, C., Zahedi, R. P., Hedberg, C., Kötting, C., Gerwert, K.
    Biochemistry 55 (31), 4375-85 (2016)
  • Mechanisms of action of Rab proteins, key regulators of intracellular vesicular transport. Goody RS, Müller MP, Wu YW.
    Biol Chem., doi: 10.1515/hsz-2016-0274 (2016)
  • First and second generation antipsychotics differentially affect structural and functional properties of rat hippocampal neuron synapses. Gottschling, C., Geissler, M., Springer, G., Wolf, R., Juckel, G. and A. Faissner.
    Neuroscience 273, 149-159 (2016)
  • The indirect neuron-astrocyte co-culture assay: an in vitro set-up for the detailed investigation of neuron-glia interactions. Gottschling, C., Dzyubenko, E., Geissler, M. and A. Faissner.
    J. Vis. Exp. (JoVE) 117, e54757, doi:10.3791/54757 (2016)
  • Nucleotide-dependent assembly of the peroxisomal receptor export complex. Grimm I, Saffian D, Girzalsky W, Erdmann R.
    Sci Rep 6, 19838. doi: 10.1038/srep19838 (2016)
  • Role of AAA+-proteins in peroxisome biogenesis and function. Grimm I, Erdmann R, Girzalsky W.
    Biochim Biophys Acta 1863(5), 828-37 (2016)
  • Selective Targeting of the KRAS G12C Mutant: Kicking KRAS When It’s Down. Hobbs G. Aaron, Wittinghofer A., and C.J. Der.
    Cancer Cell 29, 251-253 (2016)
  • Biosynthetic structure-activity relationship study of premonensin derivatives. Ismail-Ali A., Fansa E.K., Pryk N., S. Yahiaou, S.Kushnir, M.Pflieger, A. Wittinghofer and F. Schulz.
    Organic and Biomolecular Chemistry 14, 7671; DOI: 10.1039/c6ob01201a (2016)
  • Novel biochemical and structural insights into the interaction of myristoylated cargo and Unc119 and their release by Arl2/3. Jaiswal M., Fansa E.K., S.K. Koesling, T. Mejuch, H. Waldmann and A. Wittinghofer.
    J Biol Chem. 2016 Sep 23;291(39):20766-78. doi: 10.1074/jbc.M116.741827 (2016)
  • Regulation of oligodendrocyte precursor maintenance by chondroitin sulphate glycosaminoglycans. Karus, M., Czopka, T., Ulc, A., Ehrlich, M., Hennen, E., Mizhorova, M., Qamar, N., Brüstle, O., and A. Faissner.
    GLIA 64, 270-286 (2016)
  • Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Klionsky D. et al. [Autophagy Author Consortium]
    Autophagy 12, 1-222. (2016)
  • Mechanism of the intrinsic arginine finger in heterotrimeric G proteins. Mann, D., Teuber, C., Tennigkeit, S.A., Schröter, G., Gerwert, K., Kötting, C.
    PNAS 50 (113), E8041-E8050 (2016)
  • Synthesis of Lipidated Proteins. Mejuch T., Waldmann H.
    Bioconj. Chem. 27, 1771-1783 (2016)
  • dentification of pyrazolopyridazinones as PDEδ inhibitors. Papke B, Murarka S, Vogel HA, Martín-Gago P, Kovacevic M, Truxius DC, Fansa EK, Ismail S, Zimmermann G, Heinelt K, Schultz-Fademrecht C, Al Saabi A, Baumann M, Nussbaumer P, Wittinghofer A, Waldmann H, Bastiaens PI.
    Nat Commun. 2016 Apr 20;7:11360 (2016)
  • Structure of the Mis12:CENP-C complex, a central kinetochore assembly hub. Petrovic* Arsen, Keller Jenny, Yahui Liu, Katharina Overlack, Juliane John, Yoana Dimitrova, Simon Jenni, Suzan van Gerwen, Patricia Stege, Sabine Wohlgemuth, Pascaline Rombaut, Franz Herzog, Stephen C. Harrison, Ingrid.R.Vetter*, Andrea Musacchio*.
    Cell 167, 1028-1040 (2016)
  • Low-density lipoprotein receptor-related protein 1 (LRP1) – a novel modulator of neural stem and progenitor cell proliferation, survival and differentiation. Safina, D., Romeo, R., Schlitt, F., Pietrzik, C., Edenhofer, F. and A. Faissner.
    Glia 64, 1363-1380 (2016)
  • The Bisphenol A analogue Bisphenol S binds to K-Ras4B--implications for 'BPA-free' plastics. Schöpel M, Herrmann C, Scherkenbeck J, Stoll R.
    FEBS Lett. 2016 Feb;590(3):369-75. doi: 10.1002/1873-3468.12056 (2016)
  • ATP-driven processes of peroxisomal matrix protein import. Schwerter D, Grimm I, Platta HW, Erdmann R.
    Biol Chem 2016 Dec 15. pii: /j/bchm.just-accepted/hsz-2016-0293/hsz-2016-0293.xml. doi: 10.1515/hsz-2016-0293 (2016)
  • Regulation of K-Ras4B Membrane Binding by Calmodulin. Sperlich, B., S. Kapoor, H. Waldmann, R. Winter, and K. Weise.
    Biophys. J. 111; 113-122; DOI: 10.1016.bpj.2016.05.042 (2016)
  • Interface Analysis of small GTP binding protein complexes suggests preferred membrane orientations. Vetter, Ingrid R.
    Biological Chemistry; epub ahead of print: doi 10.1515/hsz-2016-0287 (2016)
  • Spatiotemporal imaging of small GTPases activity in live cells. Voss S, Krüger DM, Koch O, Wu YW.
    Proc. Natl. Acad. Sci. U. S. A. 113(50):14348-14353 (2016)
  • One-Pot N2C/C2C/N2N Ligation To Trap Weak Protein-Protein Interactions. Zhao L, Ehrt C, Koch O, Wu YW.
    Angew Chem Int Ed. 55(28): 8129-33 (2016)
  • The Chloroplast SRP Systems of Chaetosphaeridium globosum and Physcomitrella patens as Intermediates in the Evolution of SRP-Dependent Protein Transport in Higher Plants. Ziehe D, Dünschede B, Zenker M, Funke S, Nowaczyk MM, Schünemann D.
    PLoS One Nov 18;11(11):e0166818 (2016)

Publications 2015

  • Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the human Rod-Zwilch-ZW10 (RZZ) complex. Altenfeld Anika, Wohlgemuth Sabine, Annemarie Wehenkel, Ingrid R. Vetter and Andrea Musacchio.
    Acta Cryst. F71, 438-442 doi:10.1107/S2053230X15004343 (2015)
  • miRs-138 and -424 control palmitoylation-dependent CD95-mediated cell death by targeting acyl protein thioesterases 1 and 2 in CLL. Berg V., Rusch M., N. Vartak, C. Jüngst, A. Schauss, H. Waldmann, C. Hedberg, C. P. Pallasch, P. I. H. Bastiaens, M. Hallek, C.-M. Wendtner, L. P. Frenzel.
    Blood 125, 2948-2957 (2015)
  • Conditional proteolysis of the membrane protein YfgM by the FtsH protease depends on a novel N-terminal degron. Bittner LM, Westphal K, Narberhaus F.
    J Biol Chem 290:19367-19378 (2015)
  • Chemical labeling of intracellular proteins via affinity conjugation and strain-promoted cycloaddition. Chen X, Li F, Wu YW.
    Chem. Comm. 51 (92): 16537-40 (2015)
  • Molecular snapshots of the Pex1/6 AAA+ complex in action. Ciniawsky S, Grimm I, Saffian D, Girzalsky W, Erdmann R, Wendler P.
    Nat Commun 6, 7331. doi: 10.1038/ncomms8331 (2015)
  • Large-Scale Purification of Peroxisomes for Preparative Applications. Cramer J, Effelsberg D, Girzalsky W, Erdmann R.
    Cold Spring Harb Protoc 2015(9), pdb.prot083725. doi: 10.1101/pdb.prot083725 (2015)
  • Direct Modulation of Small GTPase Activity and Function. Cromm P. M., Spiegel J., T. N. Großmann, H. Waldmann.
    Angew. Chem. Int. Ed. 54/127, 13516-13537/13718-13741 (2015)
  • Chloroplast SRP54 was Recruited for Posttranslational Protein Transport via Complex Formation with Chloroplast SRP43 during Land Plant Evolution. Dünschede B, Träger C, Schröder CV, Ziehe D, Walter B, Funke S, Hofmann E, Schünemann D.
    J Biol Chem. 290:13104-13114 (2015)
  • Autophagy-related Deubiquitinating Enzymes involved in Health and Disease. El Magraoui, F., Reidick, C., Meyer, H.E. & Platta, H.W.
    Cells 4, 596-621 (2015)
  • The extracellular matrix compartment of neural stem and glial progenitor cells. Faissner, A. and J. Reinhard.
    GLIA 63(8), 1330-1349 (2015)
  • The N- and C-terminal ends of RPGR can bind to PDE6δ. Fansa E.K., O’Reilly N.J., Ismail S., and A. Wittinghofer.
    EMBO reports 16, 1583-1585 (2015)
  • Structural Characterization of LRRK2 Inhibitors. Gilsbach B.K., Messias A.C., Ito G., M. Sattler, D.R. Alessi, A. Wittinghofer, and A. Kortholt.
    J. Med. Chem. 58, 3751-3756 (2015)
  • A G-protein activation cascade from Arl13B to Arl3 and implications for ciliary targeting of lapidated proteins. Gotthardt K., Lokaj M., Koerner C., N. Falk, A. Gießl, and A. Wittinghofer.
    eLIFE, 2015, 4:e11859, DOI: 10.7554/eLIFE.11859 (2015)
  • Biochemical Large-Scale Interaction Analysis of Murine Olfactory Receptors and Associated Signaling Proteins with Post-Synaptic Density 95, Drosophila Discs Large, Zona-Occludens 1 (PDZ) Domains. Jansen F, Kalbe B, Scholz P, Fränzel B, Osterloh M, Wolters D, Hatt H, Neuhaus EM, and Osterloh S.
    Molecular & cellular proteomics: MCP 14, 2072-2084 (2015)
  • Effect of the N-terminal helix and nucleotide loading on the membrane and effector binding of Arl2/3. Kapoor S., Fansa E.K., Möbitz S., S.A. Ismail, R. Winter, A. Wittinghofer, and K. Weise.
    Biophysical Journal 109, 1619-1629 (2015)
  • The interaction of CCDC104/BARTL1 with Arl3 and implications for ciliary function. Lokaj M., Kösling S.K., Koerner C., S.M. Lange, S.E.C. van Beersum, J. van Reeuwijk, R. Roepman, N. Horn, M. Ueffing, K. Boldt, and A. Wittinghofer.
    Structure 23,1-11 (2015)
  • Erratum to: The guanine nucleotide exchange factor Vav3 regulates differentiation of progenitor cells in the developing mouse retina. Luft, V., Reinhard, J., Fischer, K.-D., Shibuya, M. and A. Faissner.
    Cell and Tissue Research 360(2), 431-433 (2015)
  • The guanine nucleotide exchange factor Vav3 regulates differentiation of progenitor cells in the developing mouse retina. Luft, V., Reinhard, J., Fischer, K.-D., Shibuya, M. and A. Faissner.
    Cell and Tissue Research 359(2), 423-440 (2015)
  • Peroxisomal Import Reduces the Proapoptotic Activity of Deubiquitinating Enzyme USP2. Reglinski K, Keil M, Altendorf S, Waithe D, Eggeling C, Schliebs W, Erdmann R.
    PLoS One 10(10), e0140685 (2015)
  • Extracellular matrix remodeling during retinal development. Reinhard, J., S. Joachim and A. Faissner.
    Special Issue: ECM in ocular development, homeostasis and disease. Exp. Eye Res. 133, 132-140 (2015)
  • Regulation of the Tumor-suppressor function of the Class III phosphatidylinositol-kinase complex by Ubiquitin and SUMO. Reidick, C., El Magraoui, F., Meyer, H.E., Stenmark, H. & Platta, H.W.
    Cancers (Basel) 7, 1-29 (2015)
  • Catalysis of GTP hydrolysis by small GTPases at atomic detail by integration of X-ray crystallography, experimental and theoretical IR spectroscopy. Rudack, T., Jenrich, S., Brucker, S., Vetter, I.R., Gerwert, K, Kötting, C.
    J. Biol. Chem. 40 (290) 24079-24090 (2015)
  • Conformational heterogeneity of the Roc domains in C.tepidum Roc-COR and implications for human LRRK2 Parkinson mutations. Rudi K., Ho F.Y., Gilsbach B.K., Pots H., A. Wittinghofer, A. Kortholt, and J.P. Klare.
    Biosci. Rep. 35, e00254 (2015)
  • Chemical Functionalization of Germanium with Dextran-Brushes for Immobilization of Proteins Revealed by ATR-FTIR Difference Spectroscopy. Schartner, J., Hoeck, N., Güldenhaupt, J., Mavarani, L., Nabers, A., Gerwert, K., and Kötting, C.
    Anal. Chem. 87, 7467-7475 (2015)
  • Ras moves to stay in place. Schmick M, Kraemer A, Bastiaens PI.
    Trends Cell Biol. Mar 7. pii: S0962-8924(15)00030-6 (2015)
  • Integration of Fourier Transform Infrared Spectroscopy, Fluorescence Spectroscopy, Steady-State Kinetics and Molecular Dynamics Simulations of Gαi1 distinguishes between the GTP Hydrolysis and GDP Release Mechanism. Schröter, G., Mann, D., Kötting, C., Gerwert, K.
    J. Biol. Chem. 290 (28) 17085-17095 (2015)
  • Cysteine-specific ubiquitination protects the peroxisomal import receptor Pex5p against proteasomal degradation. Schwartzkopff, B., Platta, H.W., Hasan, S., Girzalsky, W., Erdmann R.
    Biosci Rep 35(3), pii: e00215 (2015)
  • Revisiting the Roco G protein cycle. Terheyden S., Ho F.Y., Gilsbach B.K., A. Wittinghofer, A. Kortholt.
    Biochemical Journal 465,139-147 (2015)
  • Chemically induced dimerization: reversible and spatiotemporal control of protein function in cells. Voss S, Klewer L, Wu YW.
    Curr. Opin. Chem. Biol. 28: 194–201 (2015)
  • Locking GTPases covalently in their functional states. Wiegandt D, Vieweg S, Hofmann F, Koch D, Li F, Wu YW, Itzen A, Müller MP, Goody RS.
    Nat. Comm. 6, 7773 (2015)
  • Chemical synthesis and biological function of lipidated proteins. Yang A, Zhao L, Wu YW.
    Topics Curr. Chem. 362: 137-82 (2015)

Publications 2014

  • The pseudo GTPase CENP-M drives human kinetochore assembly. Basilico Federica, Maffini Stefano, John R. Weir, Daniel Prumbaum, Ana M. Rojas, Tomasz Zimniak, Anna De Antoni, Sadasivam Jeganathan, Beate Voss, Suzan van Gerwen, Veronica Krenn, Lucia Massimiliano, Alfonso Valencia, Ingrid R. Vetter, Franz Herzog, Stefan Raunser, Sebastiano Pasqualato, and Andrea Musacchio.
    eLife 2014;3:e02978 (2014)
  • Scaffolding by MUPP1 regulates odorant-mediated signaling in olfactory sensory neurons. Baumgart S., Jansen F., Bintig W., B. Kalbe, C. Herrmann, F. Klumpers, S. D. Köster, P. Scholz, S. Rasche, R. Dooley, N. Metzler-Nolte, M. Spehr, H. Hatt, E. M. Neuhaus.
    J. Cell Sci. 127, 2518-2527 (2014)
  • miRs-138/ -424 control CD95-mediated cell death by targeting acyl protein thioesterases 1 and 2. Berg V., Rusch M., N. Vartak, A. Schauss, H. Waldmann, C. Hedberg, M. Hallek, C. P. Pallasch, P.I.H. Bastiaens, C.-M. Wendtner, L.P. Frenzel.
    Oncology Res. and Treatm. 37, 237 (2014)
  • Predicted Incorporation of Non-Native Substrate by a Polyketide Synthase yields Bioactive Natural Product Derivatives. Bravo-Rodriguez K., Ismail-Ali A.F., Klopries S., S. Kushnir, S. Ismail, E.K. Fansa, A. Wittinghofer, F. Schulz and E. Sanchez-Garcia.
    ChemBioChem 15, 1991-1997 (2014)
  • Sequence-selective molecular recognition of the C-terminal CaaX-boxes of Rheb and related Ras-proteins by synthetic receptors. Düppe PM, Tran Thi Phuong T, Autzen J, Schöpel M, Yip KT, Stoll R, Scherkenbeck J.
    ACS Chem Biol. 2014 Aug 15;9(8):1755-63. doi: 10.1021/cb5002075 (2014)
  • The cytosolic domain of Pex22p stimulates the Pex4p-dependent ubiquitination of the PTS1-receptor. El Magraoui F, Schrötter A, Brinkmeier R, Kunst L, Mastalski T, Müller T, Marcus K, Meyer HE, Girzalsky W, Erdmann R, Platta HW.
    PLoS One. 2014 Aug 27;9(8):e105894 (2014)
  • Reaction Mechanism of Adenylyltransferase DrrA from Legionella pneumophila Elucidated by Time-Resolved Fourier Transform Infrared Spectroscopy. Gavriljuk, K., Schartner, J., Itzen, A., Goody, R.S., Gerwert, K., Kötting, C.
    J. Am. Chem. Soc. 136; 9338-9345 (2014)
  • Drosophila Ebony: a novel type of nonribosomal peptide synthetase related enzyme with unusually fast peptide bond formation kinetics. Hartwig S., Dovengerds C., C. Herrmann, B.T. Hovemann.
    FEBS J. 281, 5147-5158 (2014)
  • Chemical Biology Tools for Regulating RAS Signaling Complexity in Space and Time. Hattum H. van, Waldmann H.
    Chemistry & Biology 21, 1185-1195 (2014)
  • Neural ECM and Synaptogenesis In: A. Dityatev, B. Wehrle-Haller and A. Pitkänen Eds., Brain Extracellular Matrix in Health and Disease. Heikkinen, A., Pihlajaniemi, T., Faissner, A. and M. Yuzaki.
    Progress Brain Res. 214, 29-51 (2014)
  • Evolving models for peroxisome biogenesis. Hettema EH, Erdmann R, van der Klei I, Veenhuis M.
    Curr Opin Cell Biol. 29, 25-30. Review (2014)
  • Conformational changes at protein-protein interaction followed online with an SAW biosensor. Klumpers F., Götz U., Kurtz T., C. Herrmann, T.M.A. Gronewold.
    Sensors and Actuators B 203, 904-908 (2014)
  • Review: What vibrations tell us about GTPases. Kötting, C., Gerwert, K.
    Biol. Chem. 2015; 396(2): 131–144 (2014)
  • The membrane proteome of sensory cilia to the depth of olfactory receptors. Kuhlmann K, Tschapek A, Wiese H, Eisenacher M, Meyer HE, Hatt HH, Oeljeklaus S, Warscheid B.
    Mol Cell Proteomics 13(7):1828-43 (2014
  • The role of the hypervariable C-terminal domain in Rab membrane targeting. Li F, Yi L, Zhao L, Itzen A, Goody RS, Wu YW.
    Proc. Natl. Acad. Sci. U. S. A. 111 (7): 2572-7 (2014)
  • A modified infrared spectrometer with high time-resolution and its application for investigating fast conformational changes of the GTPase Ras. Lin, J., Gerwert, K., Kötting, C.
    Applied Spectroscopy 68 (5), 531-535 (2014)
  • A rapid and fluorogenic TMP-AcBOPDIPY probe for covalent labeling of proteins in live cells. Liu W, Li F, Chen X, Yi L, Wu YW.
    J. Am. Chem. Soc. 136 (12): 4468-71 (2014)
  • A bioorthogonal small-molecule switch system for controlling protein function in live cells. Liu P, Calderon A, Hou J, Konstantinidis G, Chen X, Li F, Banerjee S, Dehmelt L, Wu YW.
    Angew. Chem. Int. Ed. 53 (38):10049-55. (2014)
  • Crystal structure of the small G protein Arl13B from Chlamydomonas rheinhartii and implications for Joubert Syndrome. Miertzschke M., Koerner C., Spoerner M. and A. Wittinghofer.
    Biochemical Journal 457 (part 2), 301-311 (2014)
  • Insights into the Biosynthesis and Assembly of Cryptophycean Phycobiliproteins. Overkamp K. E., Gasper R., Kock K., C. Herrmann, E. Hofmann, N. Frankenberg-Dinkel.
    J Biol Chem. 289, 26691-26707 (2014)
  • The Peroxisomal Exportomer. Platta, H.W., Hagen, S. & Erdmann, R.
    In: Brocard, C. & Hartig, A. (eds.) Molecular machines involved in peroxisome biogenesis and maintenance / SpringerVerlag., 347- 370 (2014)
  • The peroxisomal receptor dislocation pathway: to the exportomer and beyond. Platta HW, Hagen S, Reidick C, Erdmann R.
    Biochemie 98, 16-28. Review (2014)
  • C2 Domains as Protein-Protein Interaction Modules in the Ciliary Transition Zone. Remans Kim, Bürger Marco, Ingrid R. Vetter and Alfred Wittinghofer.
    Cell Reports 8, 1-9 (2014)
  • Elucidating the Mode of Action of a Typical Ras State 1(T) Inhibitor. Rosnizeck I. C., Filchtinski D., Lopes R. P., B. Kieninger, C. Herrmann, H. R. Kalbitzer, M. Spoerner.
    Biochemistry 53, 3867-3878 (2014)
  • Immobilization of proteins in their physiological active state via functionalized thiol monolayers on ATR-germanium crystals. Schartner, J., Gavriljuk, K., Nabers, A., Weide, P., Muhler, M., Gerwert, K., Kötting, C.
    ChemBioChem 15, 2529 – 2534 (2014)
  • KRas Localizes to the Plasma Membrane by Spatial Cycles of Solubilization, Trapping and Vesicular Transport. Schmick, M., Vartak, N., Papke, B., Kovacevic, M., Truxius, D.C., Rossmannek, L., Bastiaens, P.I.H.
    Cell. 157(2):459-471 (2014)
  • Direct Targeting of Rab-GTPase-Effector Interactions. Spiegel J., Cromm P. M., A. Itzen, R. S. Goody, T. N. Großmann, H. Waldmann.
    Angew. Chem. Int. Ed. 53, 2498-2503 (2014)
  • Small molecule modulation of Ras signaling. Spiegel J., Cromm P. M., G. Zimmermann, T. N. Grossmann, H. Waldmann.
    Nat. Chem. Biol. 10, 613-622 (2014)
  • Subcellular fractionation and localization studies reveal a direct interaction of the fragile X mental retardation protein (FMRP) with nucleolin. Taha M. S., Nouri K., Milroy L. G., J. M. Moll, C. Herrmann, L. Brunsveld, R. P. Piekorz, M. R. Ahmadian.
    PLoS One 9, e91465 (2014)
  • The Autodepalmitoylating Activity of APT Maintains the Spatial Organization of Palmitoylated Membrane Proteins. Vartak N., Papke B., H. E. Grecco, L. Rossmannek, H. Waldmann, C. Hedberg, P. I. H. Bastiaens.
    Biophys. J. 106, 93-105 (2014)
  • The Structure of the G Domain of the Ras Superfamily. Vetter Ingrid R.
    In: Ras Superfamily Small G proteins: Biology and Mechanisms 1, pp 25-50 (2014)
  • Interaction of the Human N-Ras Protein with Lipid Raft Model Membranes of Varying Degrees of Complexity. Vogel, A., J. Nikolaus, K. Weise, G. Triola, H. Waldmann, R. Winter, A. Herrmann, and D. Huster.
    Biol. Chem. 395, 779-789 (2014)
  • Generation of an intramolecular three-color FRET probe by site-specific protein labeling. Voss S, Zhao L, Chen X, Gerhard F, Wu YW.
    J. Pep. Sci. 20 (2): 115-20 (2014)
  • Human septin isoforms and the GDP-GTP cycle. Zent E. and Wittinghofer A.
    Biol. Chem. 395 (2), 169-180 (2014)
  • Structure Guided Design and Kinetic Analysis of Highly Potent Benzimidazole Inhibitors Targeting the PDEδ Prenyl Binding Site. Zimmermann G., Schultz-Fademrecht C., P. Küchler, S. Murarka, S. Ismail, G. Triola, P. Nussbaumer, A. Wittinghofer, H. Waldmann.
    J. Med. Chem. 57, 5435-5448 (2014)

Publications 2013

  • RabGEFs are a major determinant for specific Rab membrane targeting. Blümer J, Rey J, Dehmelt L, Mazel T, Wu YW, Bastiaens P, Goody RS, Itzen A.
    J Cell Biol. 200 (3): 287-300 (2013)
  • A combined approach of quantitative interaction proteomics and live-cell imaging reveals a regulatory role for ER reticulon homology proteins in peroxisome biogenesis. David C, Koch J, Oeljeklaus S, Laernsack A, Melchior S, Wiese S, Schummer A, Erdmann R, Warscheid B, Brocard C.
    Mol Cell Proteomics 12(9), 2408-25 (2013)
  • Ras and rheb signaling in survival and cell death. Ehrkamp A, Herrmann C, Stoll R, Heumann R.
    Cancers (Basel). 2013 May 28;5(2):639-61. doi: 10.3390/cancers5020639 (2013)
  • Distinct ubiquitination cascades act on the peroxisomal targeting signal type 2 co-receptor Pex18p. El Magraoui F, Brinkmeier R, Schrötter A, Girzalsky W, Müller T, Marcus K, Meyer HE, Erdmann R, Platta HW.
    Traffic. 2013 Dec;14(12):1290-301 (2013)
  • Membrane extraction of Rab proteins by GDP dissociation inhibitor characterized using attenuated total reflection infrared spectroscopy. Gavriljuk, K., Itzen, A., Goody, R.S., Gerwert, K., Kötting, C.
    Proc. Natl. Acad. Sci. 110, No. 33, 13380-13385 (2013)
  • Mechanism of Rab1b deactivation by the Legionella pneumophila GAP LepB. Gazdag Emerich Mihai, Streller Alexandra, Ina Haneburger, Hubert Hilbi, Ingrid R. Vetter, Roger S, Goody and Aymelt Itzen.
    EMBO Rep. 14, 199-205 (2013)
  • Primary hippocampal neurons, which lack four crucial extracellular matrix molecules, display abnormalities of synaptic structures and function and severe deficits in perineuronal net formation. Geissler, M., Gottschling, C., Aguado, A., Rauch, U., Wetzel, C.H., Hatt, H. and A. Faissner.
    J. Neurosci. 33, 7742-7755 (2013)
  • Give what you can and keep what you need! Girzalsky W, Erdmann R.
    EMBO J. 32 (18), 2424-6. Review (2013)
  • The proteome of human liver peroxisomes: identification of five new peroxisomal constituents by a label-free quantitative proteomics survey. Gronemeyer T, Wiese S, Ofman R, Bunse C, Pawlas M, Hayen H, Eisenacher M, Stephan C, Meyer HE, Waterham HR, Erdmann R, Wanders RJ, Warscheid B.
    PLoS One. 2013;8(2):e57395. (2013)
  • Regulatory Proteins: How to switch a master switch. Hein C., Wittinhofer A. and V. Dötsch.
    eLife 13;2:e01159 (2013)
  • A LewisX glycoprotein screen identifies the low density lipoprotein receptor-related protein 1 (LRP1) as a modulator of oligodendrogenesis in mice. Hennen, E., Safina, D., Hausmann, U., Wörsdörfer, P., Edenhofer, F., Poetsch, A. and A. Faissner.
    J. Biol. Chem. 288, 16538-16545 (2013)
  • Pressure Modulation of Ras-Membrane Interactions and Intervesicle Transfer. Kapoor, S., A. Werkmüller, R. S. Goody, H. Waldmann, and R. Winter.
    J. Am. Chem. Soc. 135; 6149-6156 (2013)
  • Characterization of a Serine Hydrolase Targeted by Acyl-protein Thioesterase Inhibitors in Toxoplasma gondii. Kemp L.E.,. Rusch M, A. Adibekian, H.E. Bullen, A. Graindorge, C. Freymond, M. Rottmann, C. Braun-Breton, S. Baumeister, A.T. Porfetye, I.R. Vetter, C. Hedberg, and D. Soldati-Favre.
    JBC 288, 27002-27018 (2013)
  • Rab GTPase prenylation hierarchy and its potential role in choroideremia disease. Köhnke M, Delon C, Hastie ML, Nguyen UT, Wu YW, Waldmann H, Goody RS, Gorman JJ, Alexandrov K.
    PLoS One. 8 (12): e81758 (2013)
  • The dynamics of the catalytic site in small GTPases, variations on a common motif. Kötting, C., Gerwert, K.
    FEBS Letters, 587, 2025–2027 (2013)
  • Monitoring protein-ligand interactions by time-resolved FTIR difference spectroscopy. Kötting C, Gerwert K.
    Methods Mol Biol. 2013;1008:299-323. doi: 10.1007/978-1-62703-398-5_11 (2013)
  • Structural and thermodynamic characterization of Nore1-SARAH: a small, helical module important in signal transduction networks. Makbul C., Aruxandei D. C., Hofmann E., D. Schwarz, E. Wolf, C. Herrmann.
    Biochemistry 52, 1045-1054 (2013)
  • Non-native disulfide formation activates the 32-dependent heat shock response in Escherichia coli. Müller A, Hoffmann JH, Meyer HE, Narberhaus F, Jakob U, Leichert LI.
    J Bacteriol 195:2807-2816 (2013)
  • Cooperative Binding of PhoBDBD to Its Cognate DNA Sequence-A Combined Application of Single-Molecule and Ensemble Methods. Ritzefeld M., Walhorn V., Kleineberg C., A. Bieker, K. Kock, C. Herrmann, D. Anselmetti, N. Sewald.
    Biochemistry 52, 8177-8186 (2013)
  • Structural diversity of signal recognition particle RNAs in plastids. Rosenblad MA, Träger C, Schünemann D.
    Plant Signal Behav. Oct;8(10):doi: 10.4161/psb.26848 (2013)
  • Universal Method for Protein Immobilization on Chemically Functionalized Germanium Investigated by ATR-FTIR Difference Spectroscopy. Schartner, J., Güldenhaupt, J., Mei, B., Rögner, M., Muhler, M., Gerwert, K., Kötting, C.
    JACS, 135, 4079-4087 (2013)
  • Bisphenol A binds to Ras proteins and competes with guanine nucleotide exchange: implications for GTPase-selective antagonists. Schöpel M, Jockers KF, Düppe PM, Autzen J, Potheraveedu VN, Ince S, Yip KT, Heumann R, Herrmann C, Scherkenbeck J, Stoll R.
    J Med Chem. 2013 Dec 12;56(23):9664-72. doi: 10.1021/jm401291q. (2013)
  • F65 regulates and interacts with the Bloom syndrome protein in dynamic nuclear spheres –potential relevance to Alzheimer’s disease. Schrötter A, Mastalski T, Nensa FM, Neumann M, Loosse C, Pfeiffer K, Magraoui FE, Platta HW, Erdmann R, Theiss C, Uszkoreit J, Eisenacher M, Meyer HE, Marcus K, Müller T.
    J Cell Sci 126, 2480-92 (2013)
  • Macromolecular Crowding as a Suppressor of Human IAPP Fibril Formation and Cytotoxicity. Seeliger, J., A. Werkmüller, and R. Winter.
    PLoS One 8 (2013)
  • Ras GTPase activating (RasGAP) activity of the dual specificity GAP protein Rasal requires colocalization and C2 domain binding to lipid membranes. Sot B., Behrmann E., Raunser S. and A. Wittinghofer.
    PNAS 110, 111-116 (2013)
  • 7,8-dihydroxyflavone leads to survival of cultures of embryonic motoneurons by activating intracellular signaling pathways. Tsai, T., Klausmeyer, A., Leo, M., Gottschling, C., Faissner, A. and St. Wiese.
    Mol. Cell. Neurosci. 56, 18-28 (2013)
  • Tandem Orthogonal Chemically Induced Dimerization. Voss S, Wu YW.
    Chembiochem, 14 (13): 1525-1527 (2013)
  • The interplay between RPGR, PDEdelta and Arl2/3 regulate the ciliary targeting of farnesylated cargo. Wätzlich Denise, Vetter Ingrid, Katja Gotthard, Mandy Miertzschke, Yong-Xiang Chen, Alfred Wittinghofer and Shehab Ismail.
    EMBO Rep. 14, 465-472 (2013)
  • Gibbs Energy Determinants of Lipoprotein Insertion into Lipid Membranes: The Case Study of Ras Proteins. Weise, K., D. Huster, S. Kapoor, G. Triola, H. Waldmann and R. Winter.
    Faraday Discuss. 161; 549-561 (2013)
  • Rotational and Translational Dynamics of Ras Proteins upon Binding to Model Membrane Systems. Werkmüller, A., G. Triola, H. Waldmann, and R. Winter.
    ChemPhysChem 14; 3698-3705 (2013)
  • Dual lipidation of the brain-specific Cdc42 isoform regulates its functional properties. Wirth A, Chen-Wacker C, Wu YW, Gorinski N, Filippov MA, Pandey G, Ponimaskin E.
    Biochem J. 456 (3): 311-22 (2013)
  • Semisynthetic Lipidated LC3 Protein Mediates Membrane Fusion. Yang A, Li Y, Pantoom S, Triola G*, Wu YW*.
    Chembiochem, 14 (11): 1296-1300. (Cover story) (2013)
  • Boron-Based Inhibitors of Acyl Protein Thioesterases 1 and 2. Zimmermann Tobias J., Bürger Marco, Etsu Tashiro, Yasumitsu Kondoh, Nancy E. Martinez, Kristina Görmer, Sigrid Rosin-Steiner, Takeshi Shimizu, Shoichiro Ozaki, Katsuhiko Mikoshiba, Nobumoto Watanabe, Dennis Hall, Ingrid R.Vetter, Hiroyuki Osada, Christian Hedberg, and Herbert Waldmann.
    ChemBioChem 14, 115-122 (2013)
  • Small molecule inhibition of the Kras-PDEδ interaction impairs oncogenic KRAS signaling. Zimmermann G., Papke B., S. Ismail, N. Vartak, A. Chandra, M. Hoffmann, S. A. Hahn, G. Triola, A. Wittinghofer, P. I. H. Bastiaens, H. Waldmann.
    Nature 497, 638-642 (2013)

Publications 2012

  • Ubiquitination and phosphorylation of Beclin 1 and its binding partners: Tuning class III phosphatidylinositol 3-kinase activity and tumor suppression.
    Abrahamsen H, Stenmark H, Platta HW.
    FEBS Lett. 586(11):1584-91 (2012)
  • Organization and function of the Rab prenylation and recycling machinery.
    Alexandrov K., Wu Y.-W., W. Blankenfeldt, H. Waldmann, R. S. Goody.
    The Enzymes Volume 29: Protein Prenylation Part A Edited by Fuyuhiko Tamanoi, Christine A. Hrycyna and Martin O. Bergo. 147-162 (2012)
  • Tenascin C regulates proliferation and differentiation processes during embryonic retinogenesis and modulates the de-differentation capacity of Müller glia by influencing growth factor responsiveness and the extracellular matrix compartment.
    Besser, M., Jagatheaswaran, M., Schaffelke, P., Reinhard, J. and A. Faissner.
    Developmental Biology 369, 163-176 (2012)
  • Specific localization of Rabs at intracellular membranes.
    Blümer J, Wu YW, Goody RS, Itzen A.
    Biochem. Soc. Trans. 40 (6): 1421-5. Review, (2012
  • Crystal structure of the predicted phospholipase LYPLAL1 reveals unexpected functional plasticity in spite of close relationship to acyl protein thioesterases.
    Bürger Marco, Zimmermann Tobias J., Yasumitsu Kondoh, Patricia Stege, Nobomuto Watanabe, Hiroyuki Osada, Herbert Waldmann and Ingrid R. Vetter.
    Journal of Lipid Research 53, 43-50 (2012)
  • Flexible and general synthesis of functionalized phosphoisoprenoids for the study of prenylation in vivo and in vitro.
    Das D, TnimovZ, Nguyen UTT, Thimmaiah G, Lo H, Abankwa D, Wu YW, Goody RS, Waldmann H, Alexandrov K.
    EMBO J 30: 1659-1670 (2012)
  • Nanoscale distinction of membrane patches – a TERS study of Halobacterium salinarum.
    Deckert-Gaudig T., Böhme R., Freier E., Sebesta A., Merkendorf T., Popp J., Gerwert K. and Deckert V.
    Journal of BioPhotonics, 5, No. 7, 582–591 (2012)
  • Psoromic acid is a selective and covalent Rab-prenylation inhibitor targeting autoinhibited RabGGTase.
    Deraeve C, Guo Z, Bon RS, Blankenfeldt W, DiLucrezia R, Wolf A, Menninger S, Stigter EA, Wetzel S, Choidas A, Alexandrov K, Waldmann H, Goody RS, Wu YW.
    J. Am. Chem. Soc. 134 (17): 7384-91. (2012)
  • The RING-type ubiquitin ligases Pex2p, Pex10p and Pex12p form a heteromeric complex that displays enhanced activity in an ubiquitin conjugating enzyme-selective manner.
    El Magraoui F, Bäumer BE, Platta HW, Baumann JS, Girzalsky W, Erdmann R.
    FEBS J. 279(11):2060-70 (2012)
  • AAA ATPases: Structure and function.
    Erdmann R.
    Biochim Biophys Acta 1823 (1), 1. Review. (2012)
  • Detection of Lipid Raft Domains in Neutral and Anionic Langmuir Monolayers and Bilayers of Complex Lipid Composition.
    Evers F., C. Jeworrek, K. Weise, M. Tolan, and R. Winter.
    Soft Matter 8 2170-2175 (2012)
  • Molecular Requirements for peroxisomal targeting of alanine-glyoxylate Aminotransferase as an essential determinant in primary hyperoxaluria type 1.
    Fodor K, Wolf J, Erdmann R, Schliebs W, Wilmanns M.
    PLoS Biology 10 (4), 1-12. (2012)
  • Catalytic Mechanism of a Mammalian Rab•RabGAP Complex in Atomic Detail.
    Gavriljuk, K., Gazdag, E. M., Itzen, A., Kötting, C., Goody, R. S., Gerwert, K.
    Proc. Natl. Acad. Sci., 109 (52), 21348-21353 (2012)
  • Prediction of a ligand-binding niche within a human olfactory receptor by combining site-directed mutagenesis with dynamic homology modeling.
    Gelis L, Wolf S, Hatt H, Neuhaus EM, Gerwert K.
    Angew Chem Int Ed Engl 51 1274-1278 (2012)
  • A new indirect co-culture set up of mouse hippocampal neurons and astrocytes on microelectrode arrays.
    Geissler, M. and A. Faissner.
    J. Neurosci. Methods 204, 262-272 (2012)
  • Roco kinase structures explain Parkinson disease related LRRK2 mutations.
    Gilsbach Bernd K,. Ho Franz Y, Ingrid R. Vetter, Peter J.M. van Haastert, Alfred Wittinghofer, and Arjan Kortholt.
    PNAS 109, 10322-10327 (2012)
  • Chemical-biological exploration of the limits of the Ras de- and repalmitoylating machinery.
    Görmer K, Bürger M, Kruijtzer JA, Vetter I, Vartak N, Brunsveld L, Bastiaens PI, Liskamp RM, Triola G, Waldmann H.
    Chembiochem. 7;13(7):1017-23. (2012)
  • The AAA-type ATPases Pex1p and Pex6p and their role in peroxisomal matrix protein import in Saccharomyces cerevisiae.
    Grimm I, Saffian D, Platta HW, Erdmann R.
    Biochim Biophys Acta. 1823(1):150-8 (2012)
  • N-Ras forms dimers at POPC membranes.
    Güldenhaupt, J., Rudack, T., Bachler, P., Mann, D., Triola, G., Waldmann, H., Kötting, C. and Gerwert, K.
    Biophysical Journal 103, 1585-1593 (2012)
  • Structural basis for Arl3-specific release of myristoylated ciliary cargo from UNC119.
    Ismail Shehab A., Chen Yong-Xiang, Mandy Miertzschke, Ingrid R. Vetter, Carolin Koerner, and Alfred Wittinghofer.
    EMBO J. 31, 4085-3094 (2012)
  • Revealing conformational substates of lipidated N-Ras protein by pressure modulation.
    Kapoor S, Triola G, Vetter IR, Erlkamp M, Waldmann H, Winter R.
    Proc Natl Acad Sci USA 109: 460-65 (2012)
  • The Role of G-domain Orientation and Nucleotide State on the Ras Isoform-specific Membrane Interaction.
    Kapoor, S., K. Weise, M. Erlkamp, G. Triola, H. Waldmann, and R. Winter.
    Eur. Biophys. J. 41; 801-813 (2012)
  • Time-resolved FTIR spectroscopy for monitoring protein dynamics exemplified by functional studies of Ras protein bound to a lipid bilayer.
    Kötting, C., Güldenhaupt, J. and Gerwert, K.
    Chemical Physics, 396, 72-83 (2012)
  • Structure and function of the bacterial AAA protease FtsH.
    Langklotz S, Baumann U, Narberhaus F.
    BBA – Mol Cell Res 1823:40-48. (2012)
  • Exploring the Multidimensional Free Energy Surface of Phosphoester Hydrolysis with Constrained QM/MM Dynamics.
    Li, W., Rudack, T., Gerwert, K., Gräter, F. and Schlitter, J.
    J. Chem. Theory Comput. 8, 3596-3604 (2012)
  • Molecular basis of peroxisomal biogenesis disorders caused by defects in peroxisomal matrix protein import.
    Nagotu S, Kalel VC, Erdmann R, Platta HW.
    Biochim Biophys Acta. 1822(9):1326-36 (2012)
  • Pex19p contributes to peroxisome inheritance in the association of peroxisomes of peroxisomes to Myo2p.
    Ozten M, Rucktäschel R, Thoms S, Emmrich K, Krikken AM, Erdmann R, van der Klei IJ.
    Traffic 23 (7), 947-59. (2012)
  • The PtdIns3P-Binding Protein Phafin 2 Mediates Epidermal Growth Factor Receptor Degradation by PromotingEndosome Fusion.
    Pedersen NM, Raiborg C, Brech A, Skarpen E, Roxrud I, Platta HW, Liestøl K, Stenmark H.
    Traffic [Epub ahead of print] (2012)
  • Surface attached polyhistidine-tag proteins characterized by FTIR difference spectroscopy.
    Pinkerneil, P., Güldenhaupt, J., Gerwert, K. and Kötting, C.
    ChemPhysChem, 13, 2649–2653 (2012)
  • The exportomer: the peroxisomal receptor export machinery.
    Platta HW, Hagen S, Erdmann R.
    Cell Mol Life Sci 70:1393-411. Review. (2012)
  • Metal-bis(2-picolyl)amine complexes as state 1(T) inhibitors of activated Ras protein.
    Rosnizeck I. C., Spoerner M., Harsch T., S. Kreitner, D. Filchtinski, C. Herrmann, D. Engel, B. König, H. R. Kalbitzer.
    Angew. Chem. Int. Ed. 51, 10647-10651 (2012)
  • The Role of Magnesium for Geometry and Charge in GTP Hydrolysis, Revealed by Quantum Mechanics/Molecular Mechanics Simulations.
    Rudack, T., Fei X., Schlitter, J., Kötting, C. and Gerwert, K.
    Biophysical Journal 103(2) 293–302 (2012)
  • Ras and GTPase-activating protein (GAP) drive GTP into a precatalytic state as revealed by combining FTIR and biomolecular simulations.
    Rudack T, Xia F, Schlitter J, Kötting C, Gerwert K.
    PNAS Early Edition doi: 10.1073/pnas.1204333109 (2012)
  • ATP-dependent assembly of the heteromeric Pex1p-Pex6p-complex of the peroisomal matrix protein import machinery.
    Saffian D, Grimm I, Girzalsky W, Erdmann R.
    J Struct Biol 179 (2), 126-32 (2012)
  • The APP family members are key players in S-adenosylmethionine formation by MAT2A and PSEN1 gene expression – relevance for Alzheimer’s disease.
    Schrötter A, Pfeiffer K, El Magraoui, Platta H, Erdmann R, Meyer HE, Egensperger R, Marcus K, Müller T.
    Mol Cell Proteomics 11 (11), 1274-88. (2012)
  • Development of Selective, Potent RabGGTase Inhibitors.
    Stigter EA, Guo Z, Bon RS, Wu YW, Choidas A, Wolf A, Menninger S, Waldmann H, Blankenfeldt W, Goody RS.
    J. Med. Chem. 55 (19): 8330-40 (2012)
  • Time-resolved Fourier transform infrared spectroscopy of the nucleotide-binding domain from the ATP-binding Cassette transporter MsbA: ATP hydrolysis is the rate-limiting step in the catalytic cycle.
    Syberg F, Suveydis Y, Kötting C, Gerwert K, Hofmann E.
    J Biol Chem 2012 Jul 6; 287(28): 23923-31 (2012)
  • TubStain: a universal peptide-tool to label microtubule.
    Theiss C, Neumann A, Schliebs W, Erdmann R.
    Histom Cell Biol, 138 (3), 531-540 (2012)
  • Quantitative analysis of prenylated RhoA interaction with its chaperone, RhoGDI.
    Tnimov Z, Guo Z, Gambin Y, Nguyen UT, Wu YW, Abankwa D, Stigter A, Collins BM, Waldmann H, Goody RS, Alexandrov K.
    J. Biol. Chem. 287 (32): 26549-62 (2012)
  • Evolution from the prokaryotic to the chloroplast signal recognition particle pathway of higher plants: The SRP RNA is conserved in plastids of a wide range of photosynthetic organisms.
    Träger C, Rosenblad MA, Ziehe D, Garcia-Petit C, Schrader L, Kock K, Richter CV, Klinkert B, Narberhaus F, Herrmann C, Hofmann E, Aronsson H, Schünemann D.
    Plant Cell 24:4819-4836 (2012)
  • Chemical Biology of Lipidated Proteins.
    Triola G., Waldmann H., C. Hedberg.
    ACS Chemical Biology 8, 87-99 (2012)
  • Dissociation of the K Ras4B/PDEd-Complex upon Contact with Lipid Membranes: Membrane Delivery Instead of Extraction.
    Weise, K., S. Kapoor, A. Werkmüller, S. Möbitz, G. Zimmermann, G. Triola, H. Waldmann, and R. Winter.
    J. Am. Chem. Soc. 134 (2012)
  • A trapping approach reveals novel substrates and physiological functions of the essential protease FtsH in Escherichia coli.
    Westphal K, Langklotz S, Thomanek N, Narberhaus F.
    J Biol Chem 287:42962-42971 (2012)
  • Astrocytes as source of cytokines and extracellular matrix molecules.
    Wiese S., Karus, M., and A. Faissner.
    Front. Pharmacol. 3, 120. Doi:10.3389/fphar.2012.00120 (2012)
  • Liquid chromatographic analysis and mass spectrometric identification of farnesylated peptides.
    Wotske M, Wu Y, Wolters DA.
    Anal. Chem. 84 (15): 6848-55 (2012)
  • Detailed Structure of the H2PO4-Guanosine Diphoshate Intermediate in Ras-GAP Decoded from FTIR Experiments by Biomolecular Simunlations.
    Xia F, Rudack T, Cui Q, Kötting C, Gerwert K.
    Publication Date (Web): November 26, 2012, DOI: 10.1021/ja310496e (2012)
  • Inhibiting the pamiltoylation/depamitoylation cycle selectively reduces the growth of hematopoietic cells expressing oncogenic Nras.
    Xu J., Hedberg C., F. J. Dekker, Q. Li, K. M. Haigis, E. Hwang, H. Waldmann, K. Shannon.
    Blood 119, 1032-1035 (2012)
  • Direct immobilization of oxyamine-modified proteins from cell lysates. Yi L, Chen YX, Lin PC, Schröder H, Niemeyer CM, Wu YW, Goody RS, Triola G, Waldmann H.
    Chem Commun (Camb). 48 (88): 10829-31 (2012)

Publications 2011

  • Aktas M, Gleichenhagen J, Stoll R, Narberhaus F (2011) S-adenosylmethionine-binding properties of a bacterial phospholipid N-methyltransferase. J Bacteriol 193: 3473-81 
  • Bharti P, Schliebs W, Schievelbusch T, Neuhaus A, David C, Kock K, Herrmann C, Meyer HM, Wiese S, Warscheid B, Theiss C, Erdmann R (2011) PEX14 is required for microtubule-based peroxisome motility in human cells. J Cell Sci124: 1759-1768 
  • Bon RS, Guo Z, Stigter EA, Wetzel S, Menninger S, Wolf A, Choidas A, Alexandrov K, Blankenfeldt W, Goody RS, Waldmann H (2011) Structure-guided development of selective RabGGTase inhibitors. Angew Chem Int Ed Engl 50: 4957-4961 
  • Chandra A, Grecco HE, Pisupati V, Perera D, Cassidy L, Skoulidis F, Ismail SA, Hedberg C, Hanzal-Bayer M, Venkitaraman AR, Wittinghofer A, Bastiaens PI (2011) The GDI-like solubilizing factor PDEd sustains the spatial organization and signalling of Ras family proteins. Nat Cell Biol 14(2): 148-58 
  • Debelyy MO, Platta HW, Saffian D, Hensel A, Thoms S, Meyer HE, Warscheid B, Girzalsky W, Erdmann R (2011) UBp15p, an ubiquitin hydrolase associated with the peroxisomal export maschinery. J Biol Chem 286 (25): 28223-34
  • Grunau S, Lay D, Mindthoff S, Platta HW, Just W, Erdmann R (2011) The phosphoinositide-3-Kinase-Vps34p is required for pexophagy inSaccaromyces cerevisiae. Biochem J 434 (1): 161-170
  • Hedberg C, Dekker FJ, Rusch M, Renner S, Wetzel S, Vartak N, Gerding-Reimers C, Bon RS, Bastiaens PIH, Waldmann H (2011) Development of Highly Potent Inhibitors of the Ras-Targeting Human Acyl Protein Thioesterases Based on Substrate Similarity Design. Angew Chem Int Ed 50: 9832-9837 
  • Hou X, Hagemann N, Schoebel S, Blankenfeldt W, Goody RS, Erdmann KS, Itzen A (2011) A structural basis for Lowe syndrome caused by mutations in the Rab-binding domain of OCRL1. EMBO J 30: 1659-1670
  • Ismail SA, Chen YX, Rusinova A, Chandra A, Bierbaum M, Gremer L, Triola G, Waldmann H, Bastiaens PIH, Wittinghofer A (2011) Arl2-GTP and Arl3-GTP regulate a GDI-like transport system for farnesylated cargo. Nat Chem Biol 7:942-949
  • Miertzschke M, Koerner C, Vetter IR, Keilberg D, Hot E, Leonardy S, Søgaard-Andersen L, Wittinghofer A (2011) Structural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity. EMBO J 30: 4185-4197    
  • Pyka M, Wetzel C, Aguado A, Geissler M, Hatt H, Faissner A (2011) Chondroitin sulfate proteoglycans regulate astrocyte-dependent synaptogenesis and modulate synaptic activity in primary embryonic hippocampal neurons. Eur J Neurosci 33: 2187-2202 [With cover]

  • Stigter EA, Triola G, Goody RS, Waldmann H (2011) Inhibition of Rab prenylation. In: Protein Prenylation Part B, Elsevier, Amsterdam. 30: 179-203
  • Strutz-Seebohm N, Pusch M, Wolf S, Stoll R, Tapken D, Gerwert K, Attali B, Seebohm G (2011) Structural basis of slow activation gating in the cardiac I Ks channel complex. Cell Physiol Biochem 27: 443-52

  • Weise K, Kapoor S, Denter C, Nikolaus J, Opitz N, Koch S, Triola G, Herrmann A, Waldmann H, Winter R (2011) Membrane-mediated induction and sorting of K-Ras microdomain signaling platforms. J Am Chem Soc 133: 880-87
  • Wittinghofer A, Vetter IR (2011) Structure-Function relationships of the G domain, a canonical switch motif. Annu. Rev. Biochem. 80: 943-971

  • Yi L, Sun H, Itzen A, Triola G, Waldmann H, Goody RS, Wu YW (2011) One-Pot Dual-Labeling of a Protein by Two Chemoselective Reactions. Angew Chem Int Edit 50: 8287-8290

  • Yi L, Sun H, Itzen A, Triola G, Waldmann H, Goody RS, Wu YW (2011) One-pot dual-labeling of a protein by two chemoselective reactions. Angew Chem 123: 8437-8440

  • Fei, X., Rudack, T., Kötting, C., Schlitter, J. and Gerwert, K. The Specific vibrational modes of GTP in solution and bound to Ras: a detailed theoretical analysis by QM/MM simulations Phys. Chem. Chem. Phys., 13, 21451–21460 (2011)
  • Schneider, M., Wolf, S., Schlitter, J. and Gerwert, K. The structure of active opsin as a basis for identification of GPCR agonists by dynamic homology modelling and virtual screening assays. FEBS Letters 585, 3587-3592 (2011)



Publications 2010

  • Dekker FJ, Rocks O, Vartak N, Menninger S, Hedberg C, Balamurugan R, Wetzel S, Renner S, Gerauer M, Schölermann B, Rusch M, Kramer JW, Rauh D, Coates GW, Brunsveld L, Bastiaens PI, Waldmann H (2010) Small-molecule inhibition of APT1 affects Ras localization and signaling. Nat Chem Biol 6(6): 449-56
  • Chen YX, Koch S, Uhlenbrock K, Weise K, Das D, Gremer L, Brunsveld L, Wittinghofer A, Winter R, Triola G, Waldmann H (2010) Synthesis of the Rheb and K-Ras4B GTPases. Angew Chem Int Ed Engl 49:6090-95
  • Filchtinski D, Sharabi O, Rüppel A, Vetter IR, Herrmann C, Shifman JM (2010) What makes Ras an efficient molecular switch: a computational, biophysical, and structural study of Ras-GDP interactions with mutants of Raf. J Mol Biol 399: 422-435  
  • Gohlke A, Triola G, Waldmann H, Winter R (2010) Influence of the lipid anchor motif of N-ras on the interaction with lipid membranes: a surface plasmon resonance study. Biophys J 98: 2226-35

  • Ismail SA, Vetter IR, Sot B, Wittinghofer A (2010) The structure of an Arf-ArfGAP complex reveals a Ca2+-regulatory mechanism. Cell 141: 812-821 
  • Karassek S, Berghaus C, Schwarten M, Goemans CG, Ohse N, Kock G, Jockers K, Neumann S, Gottfried S, Herrmann C, Heumann R, Stoll R (2010) Ras homolog enriched in brain (Rheb) enhances apoptotic signaling. J Biol Chem 285: 33979-33991

  • Kock G, Dicks M, Heumann R, Erdmann KS, Stoll R. (2010) Sequence-specific 1H, 13C, and 15N assignment of the extended PDZ3 domain of the protein tyrosine phosphatase basophil like PTP-BL. Biomol NMR Assign 4: 199-202

  • Managadze D, Würtz C, Wiese S, Schneider M, Girzalsky W, Meyer HE, Erdmann R, Warscheid B, Rottensteiner H (2010) Identification of PEX33, a novel component of the peroxisomal docking complex in the filamentous fungus Neurospora crassa. Eur J Cell Biol 89 (12): 955-64

  • Rasche S, Toetter B, Adler J, Tschapek A, Doerner JF, Kurtenbach S, Hatt H, Meyer HE, Warscheid B, Neuhaus EM (2010) Tmem16b is specifically expressed in the cilia of olfactory sensory neurons. Chem Senses 35(3): 239-45

  • Rocks O, Gerauer M, Vartak N, Koch S, Huang ZP, Pechlivanis M, Kuhlmann J, Brunsveld L, Chandra A, Ellinger B, Waldmann H, Bastiaens PI (2010) The palmitoylation machinery is a spatially organizing system for peripheral membrane proteins. Cell 141: 458-71
  • Rosnizeck IC, Graf T, Spoerner M, Tränkle J, Filchtinski D, Herrmann C, Gremer L, Vetter IR, Wittinghofer A, König B, Kalbitzer HR (2010) Stabilizing a weak binding state for effectors in the human ras protein by cyclen complexes. Angew Chem Int Ed 49: 3830-3833
  • Sot B, Kötting C, Deaconescu D, Suveyzdis Y, Gerwert K, Wittinghofer A (2010) Unravelling the mechanism of dual-specificity GAPs. EMBO J 29: 1205-1214

  • Weinrich D, Lin, PC, Jonkheijm P, Nguyen UT, Schroder H, Niemeyer CM, Alexandrov K, Goody, RS, Waldmann, H. (2010) Oriented immobilization of farnesylated proteins by the thiol-ene reaction. Angew Chem Int Ed Engl 49: 1252-1257
  • Weise K, Triola G, Janosch S, Waldmann H, Winter R (2010) Visualizing association of lipidated signaling proteins in heterogeneous membranes - partitioning into subdomains, lipid sorting, interfacial adsorption, and protein association. Biochim Biophys Acta 1798: 1409-17  

  • Wu YW, Oesterlin LK, Tan KT, Waldmann H, Alexandrov K, Goody RS (2010) Membrane targeting mechanism of Rab GTPases elucidated by semisynthetic protein probes. Nat Chem Biol 6: 534-540 
  • Yi L, Sun H, Wu YW, Triola G, Waldmann H, Goody RS (2010) A highly efficient strategy for modification of proteins at the C terminus. Angew Chem Int Ed Engl 49: 9417-9421

  • Kötting, C., Suveyzdis, Y., Bojja, R., Metzler Nolte, N., Gerwert, K. Label-Free Screening of Drug-protein Interactions by trFTIR Spectroscopic Assays Exemplified by Ras Interactions. Applied Spectroscopy, 64 (9), 967-972 (2010)

  • Gerwert, K and Kötting, C Fourier Transform Infrared (FTIR) Spectroscopy. Encyclopedia of Life Sciences (ELS). John Wiley & Sons, Ltd: Chichester (2010)
  • Brucker, S., Gerwert, K., Kötting, C. Tyr39 of Ran preserves the Ran·GTP gradient by inhibiting GTP hydrolysis. J. Mol. Biol., 401, 1–6 (2010)

  • Begoña Sot, B., Kötting, C., Deaconescu, D., Suveyzdis, Y., Gerwert, K., Wittinghofer, A. Unraveling the mechanism of dual-specificity GAPs. The EMBO Journal, 29, 1205-1214 (2010)



Publications 2009

  • Stenzel N, Fetzer CP, Heumann R, Erdmann KS (2009) PDZ-domain-directed basolateral targeting of the peripheral membrane protein FRMPD2 in epithelial cells. J Cell Sci 122: 3374-3384
  • Tan, KT, Guiu-Rozas, E, Bon, RS, Guo, Z, Delon, C, Wetzel, S, Arndt, S, Alexandrov, K, Waldmann, H, Goody, RS, Wu, YW, Blankenfeldt, W (2009) Design, Synthesis, and Characterization of Peptide-Based Rab Geranylgeranyl Transferase Inhibitors, J Med Chem 52: 8025-8037
  • Vogel A, Reuther G, Weise K, Triola G, Nikolaus J, Tan KT, Nowak C, Herrmann A, Waldmann H, Winter R, Huster D (2009) The lipid modifications of Ras that sense membrane environments and induce local enrichment. Angew Chem Int Ed Engl 48: 8784-87
  • Weise K, Triola G, Brunsveld L, Waldmann H, Winter R (2009) Influence of the lipidation motif on the partitioning and association of N-Ras in model membrane subdomains. J Am Chem Soc 131: 1557-64
  • Völlmecke, C., Kötting, C., Gerwert, K. and Lübben, M. Spectroscopic investigation of the reaction mechanism of CopB-B, the catalytic fragment from an archaeal thermophilic ATP-driven heavy metal transporter
    FEBS Journal, 276, 6172–6186 (2009)



Publications 2008

  • Platta, H.W., Debelyy, M.O., El Magraoui, F., Erdmann, R.
    The AAA peroxins Pex1p and Pex6p function as dislocases for the ubiquitinated peroxisomal import receptor Pex5p.
    Biochem. Soc. Trans., 36, 99-104. (2008)
  • J. Güldenhaupt, Y. Adigüzel , J. Kuhlmann, H. Waldmann, C. Kötting, K. Gerwert
    Secondary structure of lipidated Ras bound to a lipid bilayer
    FEBS Journal 23, 5910-5918 (2008)

  • J. Güldenhaupt, Y. Adigüzel , J. Kuhlmann, H. Waldmann, C. Kötting, K. Gerwert
    Secondary structure of lipidated Ras bound to a lipid bilayer
    FEBS Journal 23, 5910-5918 (2008)

  • G .Triola, L. Brunsveld, H. Waldmann
    Racemization-free synthesis of S-alkylated cysteines via thiol-ene reaction
    J. Org. Chem.73, 3646-3649 (2008)

  • T. Govindaraju, P. Jonkheijm, L. Gogolin, H. Schroeder, C. F. W. Becker, C. M. Niemeyer, H. Waldmann
    Surface immobilization of biomolecules by click sulfonamide reaction
    ChemComm 10, 3723-3725 (2008)

  • Z. Guo, Y.-W. Wu, D. Das, C. Delon, J. Cramer, S. Yu, S. Thuns, N. Lupilova, H. Waldmann, L. Brunsveld, R. S. Goody, K. Alexandrov, W. Blankenfeldt
    Structures of RabGGTase-substrate/product complexes provide insights into the evolution of protein prenylation
    Embo J. 27, 2444-2456 (2008)

  • P. Jonkheijm, D. Weinrich, H. Schroeder, C. M. Niemeyer, H. Waldmann
    Chemical Strategies for Generating Protein Biochips
    Angew. Chem. 120, 9762-9792 (2008)
    Angew. Chem. Int. Ed. 47, 9618-9647 (2008)

  • P. Jonkheijm, D. Weinrich, M. Köhn, H. Engelkamp, P.C.M. Christianen, , J. Kuhlmann, J.C. Maan3, D. Nüsse, H. Schroeder, R. Wacker, R. Breinbauer, C.M. Niemeyer, H. Waldmann

    Angew. Chem. 120, 4493-4496 (2008)
    Angew. Chem. Int. Ed. 47, 4421-4424 (2008)

  • Z. Guo, Y.-W. Wu, K.-T. Tan, R. S. Bon, E. Guiu-Rozas, C. Delon, U. T. Nguyen, S. Wetzel, S. Arndt, R. S. Goody, W. Blankenfeldt, K. Alexandrov, H. Waldmann
    Development of Selective RabGGTase Inhibitors and Crystal Structure of a RabGGTase-Inhibitor Complex
    Angew. Chem. 120, 3807-3810 (2008)
    Angew. Chem. Int. Ed. 47, 3747-3750 (2008)

  • Rauch A, Leipelt M, Russwurm M, Steegborn, C.
    Crystal structure of the guanylyl cyclase Cya2.
    Proc Natl Acad Sci USA 105, 15720-15725 (2008)
  • B. Warscheid, S. Brucker, A. Kallenbach, H. E. Meyer, K. Gerwert, C. Kötting
    Systematic approach to group-specific isotopic labeling of proteins for vibrational spectroscopy. Vibr. Spectrosc.
    in press (DOI: 10.1016/j.vibspec..11.003) (2008)
  • Barbour, J., Neuhaus, E. M., Piechura, H., Stoepel, N., Brunert, E. M. D., Mashukova, A., Sitek, B., Stühler, K., Meyer, H. E., Hatt, H., Warscheid, B.
    Novel insight into stimulus-induced plasticity of the olfactory epithelium in Mus musculus by quantitative proteomics
    In revision.



Publications 2007

  • Chakrabarti, P., Daumke, O., Suveyzdis, Y., Kötting, C., Gerwert, K., Wittinghofer, A
    Insight into catalysis of a unique GTPase reaction by a combined biochemical and FTIR approach
    J. Mol. Biol. (367), 983-995 (2007)
  • Y.-W. Wu, K.-T. Tan, H. Waldmann, R. S. Goody, K. Alexandrov
    Interaction analysis of prenylated Rab GTPase with Rab escort protein and GDP dissociation inhibitor explains the need for both regulators
    PNAS, 104, 12294-12299, 2007
  • U. T. T. Nguyen, J. Cramer, J. Gomis, R. Reents, M. Gutierrez-Rodriguez, R. S. Goody, K. Alexandrov, H. Waldmann
    Exploiting the Substrate Tolerance of Farnesyltransferase for Site- Selective Protein Derivatization
    ChemBioChem, 8, 408-423, 2007
  • F. Bringezu, M. Majerowicz, S. Wen, G. Reuther, K.-T. Tan, J. Kuhlmann, H. Waldmann, D. Huster
    Membrane Binding of a Lipidated N-Ras Protein Studied in Lipid Monolayers
    Eur Biophys J, 36, 491-498, 2007
  • Saric M., Zhao X., Körner C., Nowak C., Kuhlmann J., and Vetter I. R.
    Structural and biochemical characterization of the Importin-ß-Ran GTP RanBD1 complex
    FEBS Letters 581: 1369-1376 (2007). A3, A7
  • Wiese, S., Gronemeyer, T., Ofman, R., Kunze, M., Grou, C. P., Almeida, J. A., Eisenacher, M., Stephan, C., Hayen, H., Schollenberger, L., Korosec, T., Waterham, H. R., Schliebs, W., Erdmann, R., Berger, J., Meyer, H. E., Just, W., Azevedo, J. E., Wanders, R. J., Warscheid, B.
    Proteomic characterization of mouse kidney peroxisomes by tandem mass spectrometry and protein correlation profiling
    Mol. Cell. Proteomics 6, 2045-57



Publications 2006

  • Kötting, C., Blessenohl, M., Suveyzdis, Y., Goody, R.S., Wittinghofer, A., Gerwert, K
    A phosphoryl transfer intermediate in the GTPase reaction of Ras in complex with its GTPase-activating protein
    Proc. Natl. Acad. Sci USA (103), 13911-13916 (2006) COVER story
  • O. Pylypenko, A. Rak, T. Durek, S. Kushnir, B. E. Dursina, N. H. Thomae, A. T. Constantinescu, L. Brunsveld, A. Watzke, H. Waldmann, R. S. Goody, K. Alexandrov
    Structure of doubly prenylated Ypt1: GDI complex and the mechanism of GDI mediated Rab recycling
    Embo J., 25, 13-23, 2006
  • Dantas de Araújo, J. M. Palomo, J. Cramer, M. Köhn, H. Schröder, R. Wacker, C. M. Niemeyer, K. Alexandrov, H. Waldmann
    Diels-Alder Ligation and Surface Immobilization of Proteins
    Angew. Chem./Angew. Chem. Int. Ed., 118/ 45, 302-307/296-301, 2006
  • Watzke, M. Köhn, M. Gutierrez-Rodriguez, R. Wacker, H. Schröder, R. Breinbauer, J. Kuhlmann, K. Alexandrov, C. M. Niemeyer, R. S. Goody, H. Waldmann
    Site-Selective Protein Immobilization by Staudinger Ligation
    Angew. Chem. Int. Ed., 45, 1408-1412, 2006
  • L. Brunsveld, J. Kuhlmann, K. Alexandrov, A. Wittinghofer, R. S. Goody, H. Waldmann
    Lipidated Ras and Rab Peptides and Proteins – Synthesis, Structure and Function (Review)
    Angew. Chem. Int. Ed. 45, 6622-6646, 2006
  • G. Reuther, K.-T. Tan, J. Köhler, C. Nowak, A. Pampel, K. Arnold, J. Kuhlmann, H. Waldmann, D. Huster
    Structural Model of the Membrane-Bound C-Terminus of Lipid-Modified Human N-Ras Protein
    Angew. Chem. Int. Ed., 45; 5387-5390, 2006
  • Dursina, R. Reents, M. Thutewohl, A. Veligodsky, A. Kalinin, V. Evstifeev, D. Ciobanu, S. E. Szedlacsek, H. Waldmann, R. S. Goody, K. Alexandrov
    Identification and Specificity Profiling of Protein Prenyltransferase Inhibitors Using New Fluorescent Phosphoisoprenoids
    J. Am. Chem. Soc., 128, 2822-2835, 2006
  • C. Nicolini, J. Baranski, S. Schlummer, J. Palomo, M. Lumbierres-Burgues, M. Kahms, J. Kuhlmann, S. Sanchez, E. Gratton, H. Waldmann, R. Winter
    Visualizing Association of N-Ras in Lipid Microdomains: Influence of Domain Structure and interfacial Adsorption
    J. Am. Chem. Soc, 128, 192-201, 2006
  • G. Reuther, K.-T. Tan, A. Vogel, C. Nowak, K. Arnold, J. Kuhlmann, H. Waldmann, D. Huster
    The Lipidated Membrane Anchor of Full Length N-Ras Protein Shows an Extensive Dynamics as Revealed by Solid-State NMR Spectroscopy
    J. Am. Chem. Soc., 128, 13840-13846, 2006
  • A. Gottlieb, C. Grunwald, C. Nowak, J. Kuhlmann, H. Waldmann
    Intein-Mediated in vitro Synthesis of Lipidated Ras Proteins
    ChemComm., 260-262, 2006
  • Y.-W. Wu, H. Waldmann, R. Reents, F. H. Ebetino, R. S. Goody, K. Alexandrov
    A Protein Fluorescence Amplifier: Continuous Fluorometric Assay for Rab Geranylgeranyltransferase
    ChemBioChem, 7, 1859-1861, 2006
  • A. Watzke, M. Gutierrez-Rodriguez, M. Köhn, R. Wacker, H. Schröder, R. Breinbauer, J. Kuhlmann, K. Alexandrov, C. M. Niemeyer, R. S. Goody, H. Waldmann
    A Generic Building Block for C- and N-Terminal Protein-Labeling and Protein-Immobilization
    Bioorg. Med. Chem., 14, 6288-6306, 2006
  • A. Meister, C. Nicolini, H. Waldmann, J. Kuhlmann, A. Kerth, R. Winter, A. Blume
    Insertion of lipidated Ras Proteins Into lipid monolayers studied by infrared reflection absorption spectroscopy (IRRAS)
    Biophys. J., 91, 1388-1401, 2006
  • L. Brunsveld, J. Kuhlmann, H. Waldmann
    Synthesis of Palmitoylated Ras-Peptides and –Proteins
    Methods, 40, 151-165, 2006
  • A. Ghosh, G.J.K. Praefcke, L. Renault, A. Wittinghofer, C. Herrmann
    How Guanylate Binding Proteins achieve assembly-stimulated processive cleavage of GTP to GMP
    Nature 440, 101-104 (2006)
  • Neuhaus, E. M., Mashukova, A., Barbour, J., Wolters, D., Hatt, H.
    Novel function of ß-arrestin2 in the nucleus of mature spermatozoa
    J. Cell Sci. 119: 3047-3056 (2006)



Publications 2005

  • O. Rocks, A. Peyker, M. Kahms, P. J. Verveer, C. Koerner, M. Lumbierres, J. Kuhlmann, H. Waldmann, A. Wittinghofer, P. I. H. Bastiaens
    An Acylation Cycle Regulates Localization and Activity of Palmitoylated Ras Isoforms
    Science, 307, 1745-1752, 2005
  • L. Brunsveld, A. Watzke, T. Durek, K. Alexandrov, R. S. Goody, H. Waldmann
    Synthesis of Functionalized RabGTPases by Combination of Solution and Solid Phase Lipopeptide Synthesis with Expressed Protein Ligation
    Chem. Eur. J., 11, 2756-2772, 2005
  • R. Reents, M. Wagner, St. Schlummer, J. Kuhlmann, H. Waldmann
    Synthesis and Application of Fluorescent Ras-Proteins for Live Cell Imaging
    ChemBioChem 6, 86-94, 2005
  • Watzke, L. Brunsveld, T. Durek, K. Alexandrov, A. Rak, R.S. Goody, H. Waldmann
    Chemical Biology of Protein Lipidation: Semi-Synthesis and Structure Elucidation of Prenylated RabGTPases
    Org. Biomol. Chem., 3, 1157-1164, 2005
  • E. Dursina, R. Reents, A. Niculae, A. Veligodsky, R. Breitling, K. Pyatkov, H. Waldmann, R. S. Goody, K. Alexandrov
    A genetically encodable microtag for chemo-enzymatic derivatization and purification of recombinant proteins
    Protein Expression and Purification, 39, 71-81, 2005
  • R. S. Goody, T. Durek, H. Waldmann, L. Brunsveld, K. Alexandrov
    Application of Protein Semi-synthesis for the Construction of Functionalised Posttranslationally Modified RabGTPases
    Methods in Enzymology, 403, 29-42, 2005
  • Rak, O. Pylypenko, T. Durek, A. Watzke, H. Waldmann, R.S. Goody, K. Alexandrov
    Protein semi-synthesis and its application to studies of structure and function of GTPases
    Biopolymers, 80, 490, 2005
  • M. Spoerner, A. Nuehs, P. Ganser, C. Herrmann, A. Wittinghofer, H. R. Kalbitzer
    Conformational states of Ras complexed with the GTP-analogs GppNHp or GppCH2p: implications for the interaction with effector proteins
    Biochemistry 44, 2225-2236 (2005)
  • S. Wohlgemuth, C. Kiel, A. Krämer, L. Serrano, A. Wittinghofer, C. Herrmann
    Recognizing and Defining true Ras Binding Domains I: Biochemical Analysis
    J. Mol. Biol. 348, 741-758 (2005)



Publications 2004

  • Chakrabarti, P., Suveyzdis, Y., Wittinghofer, A., Gerwert, K.
    Fourier Transform Infrared Spectroscopy on the Rap·RapGAP Reaction, GTPase Activation without an Arginine Finger
    Journal of Biological Chemistry (279), 46226-46233 (2004)
  • C. Peters, A. Wolf, M. Wagner, J. Kuhlmann, H. Waldmann
    The Cholesterol Membrane Anchor of the Hedgehog Protein Confers Stable Membrane Association to Lipid-Modified Proteins
    Proc. Natl. Ac. Sci, 101, 8531-8536, 2004
  • R. Reents, M. Wagner, J. Kuhlmann, H. Waldmann
    Synthesis and Application of Fluorescence-Labeled Ras-Proteins for Live-Cell Imaging
    Angew. Chem. / Angew. Chem. Int. Ed., 116 / 43, 2765-2768 / 2711-2714, 2004
  • S. Janosch, C. Nicolini, B. Ludolph, C. Peters, M. Völkert, Th. L. Hazlet, E. Gratton, H. Waldmann, R. Winter
    Partitioning of Dual-lipidated Peptides into Membrane Microdomains – Lipid Sorting vs. Peptide Aggregation
    J. Am. Chem. Soc, 126, 7496-7503, 2004
  • T. Durek, K. Alexandrov, R. S. Goody, A. Hildebrand, I. Heinemann, H. Waldmann
    Synthesis of Fluorescently Labeled Mono- and Doubly Prenylated Rab7 GTPase
    J. Am. Chem. Soc.,, 126, 16368-16378, 2004
  • L. Soulère, C. Aldrich, O. Daumke, R. Gail, L. Kissau, A. Wittinghofer, H. Waldmann
    Synthesis of GTP-Derived Ras-Ligands
    ChemBioChem, 10, 1448-1453, 2004
  • O. Müller, E. Gourzoulidou, M. Carpintero, I.-M. Karaguni, A. Langerak, C. Herrmann, T. Möröy, H. Waldmann
    "Identification of potent Ras signaling inhibitors by pathway-selective phenotype-based screening"
    Angew. Chem. Int. Ed. 43, 450-454 (2004)
  • H. Waldmann, I.-M. Karaguni, M. Carpintero, E. Gourzoulidou, C. Herrmann, C. Brockmann, H. Oschkinat, O. Müller
    "Sulindac-derived Ras pathway inhibitors target the Ras-Raf interaction and downstream effectors in the Ras pathway"
    Angew. Chem. Int. Ed. 43, 454-8 (2004)